Pdf serial dilution lab

Dilution tables in your lab book can be madeup ahead of lab. A small amount of serum or solute can be serially diluted by transferring aliquots to. Single dilutions background stock solutions are constantly diluted in biological lab settings. Lab report 1 isolation, identification and use of serial. Dilutions and concentrations introductory chemistry. Introduction to electromagnetic waves pdf serial dilution lab report paper mame romset version 0. These standards are used to create what is known as a calibration curve, or standard curve. For example, if you want to accurately quantitate the amount of bacteria in a laboratory culture, you need to dilute the. Prepare serial dilutions of the broth culture as shown in the figure from a previous lab exercise isolation of pure cultures. Lab 17 serial dilution free download as powerpoint presentation.

In this basic laboratory experiment, 100 ml 1 m solution of nacl and water was calculated and later mixed, before 1 ml of it was dropped into a 25 ml flask and was filled with water. Eight steps to method validation in a clinical diagnostic. All wells in the serial dilution plate contain 150 l of liquid when the run is completed. Producing a serial dilution for a glucose calibration curve or in microbiology for bacterial growth. It may be necessary to assay one or two serial dilutions of the initial antigen test solution to ensure that at least one of the dilutions can be accurately measured.

Pdf lab report of microbiology akbar haqi academia. In an experiment involving concentration curves, you can use a serial dilution to create a series of solutions with dilutions of 1, 1. The dilution of a sample in a diluent the liquid used to dilute the sample can be calculated as. Laboratory procedure there is only six hours for each experiment. Medical personnel commonly must perform dilutions for iv solutions. Analyzing your uncertainties allows you to identify the weaknesses in your systems. This is part of the required practicals for alevel biol. To complete a tenfold dilution, the ratio must be 1. In the microbiology lab biology 201 the students perform a three step 1. The importance of serial dilution and colony counting is reflected by the number of. The source of dilution material for each step comes from.

C1v1c2v2 percent solutions parts per hundred molar solutions unitmmolesl. Lab math solutions, dilutions, concentrations and molarity aphl. The dilution error although present in the serial dilution experiment is not an input. Serial dilution lab report microbiology lab report sample. Oct 26, 2019 serial dilution lab report pdf for many quantification assays, a set of standards must be run alongside test samples in order to calibrate an experiment properly. From this curve, the quantity of target in a sample can be calculated. Materialstapwater or pond water sample nutrient agar plates dilution water blanks 9 ml pipettes 1 ml, sterile pipette bulb or mechanical pump glass spreaders alcohol in a beaker vortex mixer marking pen methods 1. Fundamental lab skills serial dilution, dilutions and solution prepname. Molarity, dilutions, and preparing solutions lab r. Be familiar with theory of operation of instrument before lab. Solutions can be diluted by removing a known amount from a solution of known concentration and mixing it with an amount of solvent to reestablish the original volume. Aids clinical trials group virology manual for hiv.

Mic can even be performed on normally sterile body fluids without isolating and identifying the pathogenic microorganisms. Typically, the dilution factor remains constant for each dilution, resulting in an exponential decrease in concentration. Booker microbiology 2310 lab report 3 serial dilutions october 15, 2017 introduction. This results in a solution of a molarity proportional to the amount removed divided by the total volume. A series of five dilutions is made of this first dilution by diluting it 110, rediluting 110, and then three times more, each resulting solution then. But equally the equipment has a detection limit so we cant diluteit to much, or. Serial dilution required practical revision for biology. The following formula can easily be used to carry out a simple dilution. A serial dilution consists of a series of stepwise dilutions performed in a. Upon completion of this lab, students will be able to. Pdf titration of microorganisms in infectious or environmental samples is a corner. A serial dilution has multiple stages and is most practical when the change in concentration is very great. To make our dilution we would use 200ul of serum and 200 ul, or 800 ul of water. A serial dilution is a series of dilutions made sequentially, using the same dilution factor for each step.

Serial dilutions of samples are made using emem10 as the diluent in 8well labtek slides. Materialstapwater or pond water sample nutrient agar plates dilution water blanks 9 ml pipettes 1 ml, sterile pipette bulb or mechanical pump glass spreaders alcohol. Serial dilutions and standard curves with a microplate. Lab math solutions, dilutions, concentrations and molarity. Lab 17 serial dilution colony forming unit life sciences. This results in a solution of a molarity proportional to the amount removed divided by. A series of five dilutions is made of this first dilution by diluting it 110, rediluting 110, and then three times more, each resulting solution then being a 110 dilution of the previous one in the series. As shown in the illustration below, it begins when 1 ml of the bacterial sample is added to 9 ml. In simple words, serial dilution is the process of stepwise dilution of a solution with an associated dilution factor.

The usage of burette was involved in the second experiment, disposing it up to. Repeat steps 1 through 4 to produce 3 independent serial dilutions of the control template. Abstract this lab will have a serial dilution of 1 moll hcl to 0. Serial dilution lab in this unit we will be discussing measurements of concentration that us e both very big and very small numbers. For medical laboratory technology students immunology and serology selamawit debebe. Specimens exceeding the high limit are automatically diluted 1. In this short dry lab exercise, students perform serial dilutions using seed beads. A serial dilution is a series of simple dilutions which amplifies the dilution factor quickly. Dilutions in the range 101 110 to 108 1100,000,000 are generally used, although with particular types of samples the range of dilutions can be restricted.

A microbe is an organism that is microscopic and submicroscopic, which is too tiny to be viewed under a naked eye. The young scientist program exists to promote science literacy among students in grades k12, encourage the pursuit of careers in stem by introducing participants to areas of scientific study, provide reliable personal and academic mentorship to students to help them in their pursuit of stem careers, and develop participants laboratory and. For example, blood or cerebrospinal fluid containing an infecting microorganism can be added to tubes containing various dilutions of an. Serial dilution lab report conclusion serial dilution lab purpose worksheet often the standard which is given to you in thelab is far to strong for the experiment and it needs to be diluted. A serial dilution is the dilution of a sample, in 10fold dilutions. Serial dilutions reduce the concentration of a sample in small steps or fractions. The serial dilution assay is a standard microbiological method for determining the density of organisms in a solution. Using a serial dilution experiment to estimate the. This exercise is intended to familiarize us with using scientific notation to express big and small number easily and the process of using serial dilutions to make concentrations of known values. This exercise helps students gain skill at performing dilutions without using reagents, bacterial cultures, or viral cultures, while being able to visualize the process. Serial dilution is a common technique used in many immunologic procedures. The second rule of dilutionsconcentrations is an obvious one.

Log dilutions a log dilution is a tenfold dilution, meaning the concentration is decreased by a multiple of ten. But equally the equipment has a detection limit so we cant diluteit to much, or if it is too diluted the experiment might notwork. Serial dilution to begin, you must know how to calculate dilution. This webinar is intended to give a brief introduction into the mathematics of making solutions commonly used in a research setting. For example, for water that is not turbid, the maximal dilution needed is 106 because we know that if there were 107 or more. A dilution is prepared by adding a specific amount of a concentrate to water to obtain a new volume and concentration. Introduction the accurate identification of microorganisms is vitally important in several areas of microbiology including. Prepare dilutions of test antigen solutions in blocking buffer. In biology, serial dilution is often associated with reducing the concentration. One common unit of concentration is molarity m, which is equal to moles of solute per liters of solution. Serial dilution definition, formula, calculator, procedure, uses.

Accurate and consistent serial dilutions made easy with andrew. Serial dilution a serial dilution is simply a series of simple dilutions which amplifies the dilution factor quickly beginning with a small initial quantity of material i. Lab reports are due the beginning of the following lab period the week after the lab is completed. Isolation, identification and use of serial dilutions to estimate cell numbers aim 1. Dilutions and concentrations introductory chemistry 1st. As the name implies, serial dilutions of a sample are spread onto the surfaces of agar plates. Probably the most common application in biology of this technique is in the measurement of the concentration of a compound in solution. For example, one in perhaps a million trypanosomes may.

Serial dilutions and standard curve biology libretexts. For medical laboratory technology students immunology and serology selamawit debebe alemaya university in collaboration with the ethiopia public health training initiative, the carter center, the ethiopia ministry of health, and the ethiopia ministry of education 2004. Sep 02, 2012 issues should be addressed before beginning the lab. Serial dilutions involve diluting a stock or standard solution multiple times in a row. In the research laboratory, this data is important in standardizing and.

A simple method is presented to estimate the microbial counts obtained with the serial dilution technique for microorganisms that can grow on bacteriological media and develop into a colony. The source of dilution material for each step comes. Be sure to mix the nutrient broth tubes before each serial transfer. Dilution is a series of sequential dilution of a substance in. Uncertainty in dilutions without exploring the uncertainties of our dilutions, we cannot have confidence in our calibrations and therefore our results. Preparing a stock solution ing a stock solution of known concentration ametric flask, g 59. It may be tempting to think that vc is the amount of the concentrated. Serial dilutions tube method principle serial dilution is a. The concentration factor is the initial volume divided by the final solution volume.

Sample lab 1 report 2 lab report 1 sample serial dilution of a bacterial culture introduction genetic analysis often requires the measurement of the frequency of events that are not likely to occur except in a large sample. Serial dilution of hcl aq from a concentration of 1 moll to 0. For longer periods, store the serum at lab settings. All three bacterial plate count methods described in lab require you to serially dilute your samples until you have 30300 colony forming units cfu on the plate. Pdf estimation method for serial dilution experiments. Determine the mass of solute needed to make at %wv solution. For most assay systems, test solutions containing 1 to 100 ngml of antigen can be accurately measured. It would be more difficult to do a practical dilution of 1moll to. Protocol for using serial dilutions in the microbiology lab. Estimation method for serial dilution experiments core. It is critical that you master both serial and simple single dilutions.

Our final concentration then is equal to the stock concentration divided by our dilution factor of ten to the sixth power. While you may already make solutions in the lab by following recipes, we hope this webinar will help you understand the concepts involved so that you can. In this lab you will practice preparing solutions of different concentrations. Preparation of samples and dilutions, plating and subculture. For example, a tenfold serial dilution could result in the following concentrations. View full document serial dilution of hcl aq from a concentration of 1 moll to 0. May 27, 2020 serial dilution, as the name suggests, is a series of sequential dilutions that are performed to convert a dense solution into a more usable concentration. Serial dilution simulation lab the american biology teacher. Serial dilution protocol pdf it is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. Serial dilution simulation lab the american biology. The source of dilution material for each step comes from the diluted material of the previous. The amount of solute that is dissolved in a given quantity of solvent is expressed as the concentration of the solution. These are frequently used to evaluate the students understanding of the material.

May 01, 2010 serial dilution is often a difficult concept for students to understand. Simple dilution dilution factor method based on ratios a simple dilution is one in which a unit volume of a liquid material of interest is combined with an appropriate volume of a solvent liquid to achieve the desired concentration. The protocol sets up the serial dilution plate as follows. If a manual dilution is required, dilute the specimen in normal saline, and multiply the. The dilution factor is the total number of unit volumes in which your material will be.

In this lab, a serial dilution will be made of a sample of bacteria, and then those dilutions will be used to culture bacteria in order to estimate their numbers. The usage of burette was involved in the second experiment, disposing it up to 8. This will give 7 control template dilutions in the range of 1001. Six data points were collected for hba1c by serial dilution starting at a concentration of 14 percent and diluting the sample to concentrations of 10, 9, 6, and 5 percent. This process may be repeated as many times as necessary to achieve the desired solution. In order to calculate dilutions of solutions the equation. Manual homogenisation is also permissible, samples must be manually homogenised. The lab report and marks can be viewed, but labs are not returned. Serial dilution required practical revision for biology and. Preparation of samples and dilutions, plating and subculture gov. Mar 01, 2018 a serial dilution was performed to determine the linearity of the method throughout the reportable range. Serial dilution activity word pdf salol lab titration of vitamin c with iodine pdf. Mar 29, 2019 extend this procedure to perform longer serial dilutions.